Microbiology
Mahsa Amirani; Shiva Khalil moghadam; Kumarss Amini
Volume 27, Issue 1 , May and June 2020, , Pages 48-54
Abstract
Backgeround: Klebsiella pneumoniae is an opportunistic pathogen and is one of the most important bacteria involved in hospital infections.The broad spectrum of beta-lactatmase is increasing worldwide. Antibiotics of β-lactams are among the most common antimicrobial agents in controlling and treating ...
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Backgeround: Klebsiella pneumoniae is an opportunistic pathogen and is one of the most important bacteria involved in hospital infections.The broad spectrum of beta-lactatmase is increasing worldwide. Antibiotics of β-lactams are among the most common antimicrobial agents in controlling and treating bacterial infections. The aim of this study was to determine the CTX-M-2, CTX-M-8 and CMY-2 genes in Klebsiella pneumoniae strains producing broad-spectrum ß-lactamase isolated from Tehran hospitals.Methods: In present study, 60 samples were collected from different hospitals in Tehran and were identified by biochemical tests. Antibiotic susceptibility test was performed using CLSI absolute diffusion method. Also, To detect beta-lactamases, double-synergistic test was used. the multiplex-PCR was utilized to detect beta-lactamase genes.Results: In this study, the highest resistance to ampicillin antibiotics was determined .Also, the highest sensitivity to antibiotic amikacin was .Among 60 samples, 33 samples contained the CMY-2 gene, 8 samples contained the CTX-M-2 gene and 25 samples contained the CTX-M-8 gene.The results of the investigationon the basis of SPSS software showed that the relationship between the studied genes and antibiotic resistance is significant.(p value